Anti-munc-18-1

Not available outside of the UK & Ireland.

Application

Anti-Munc-18-1 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Munc-18-1 functions in synaptic vesicle exocytosis. It modulates neurotransmission by associating with syntaxin 1. Munc-18-1 binds to both syntaxin 1A and 1B isoforms, inhibiting the binding of t-soluble N-ethylmaleimide-sensitive factor-attached protein receptor (SNARE) synaptobrevin/vesicle-associated membrane protein (VAMP) and SNAP25 to syntaxin 1 and preventing SNARE assembly. Munc-18 is required for neurotransmitter secretion from synaptic vesicles throughout development and has been implicated in axonal branching in hippocampal neurons.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Munc-18-1 (67 kDa) is an abundant neuronal protein that tightly binds to the synaptic fusion protein syntaxin. It is primarily expressed in neuronal tissues. Both Munc-18-1 and 2 isoforms bind tightly to syntaxins 1A, 2, and 3.

Immunogen

synthetic peptide corresponding to amino acids 577-594 located at the C-terminus of rat Munc-18-1 conjugated to KLH. This sequence is identical in mouse, chicken, bovine, and human Munc-18-1. It does not share homology with the Munc-18-2 and Munc-18-3 isoforms.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.