Anti-phospho-c-jun (phosphoserine 63),*d

Not available outside of the UK & Ireland.

Application

Anti-phospho-c-Jun (pSer63) antibody produced in rabbit has been used in microarray analysis to identify the changes in the level of cellular proteins in cells inoculated with Equine influenza virus.

Biochem/physiol Actions

The protooncogene c-Jun encodes a member of the jun family that forms a component of the transcription factor AP-1 (Activator Protein-1). It dimerizes with a member of the Fos family via the leucine-zipper motif to form AP-1, a factor involved in binding to TPA (12-O-tetradecanoylphorbol 13-acetate) response element (TRE) of various genes that include human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. The dimers formed are involved in the activation of AP-1 dependent genes. AP-1 regulates the transcription of mammalian DDI (DNA-damage-inducible) genes.

Immunogen

synthetic phosphoserine⁶³ peptide corresponding to residues around Ser⁶³ of human c-Jun, conjugated to KLH.

Physical form

Solution in 10 mM HEPES sodium, pH 7.5, containing 150 mM sodium chloride, 100 µg/mL bovine serum albumin, and 50% glycerol.

Specificity

The antibody does not react with the corresponding phosphorylated form of JunD or JunB.