Osteocalcin; Bovine Bone

Not available outside of the UK & Ireland.

General description

Single chain vitamin K-dependent protein produced by osteoblasts. Osteocalcin purified from bovine bone. Binds to phospholipid vesicles in the presence of Ca²⁺ (K_d = 6 µM). Also binds hydroxylapatite. A good marker of bone turnover. May play a role in bone mineralization, bone resorption, and bone formation.

Single chain vitamin K-dependent protein produced by osteoblasts. Osteocalcin purified from bovine bone. Binds to phospholipid vesicles in the presence of Ca²⁺ (K_d = 6 µM). Also binds hydroxylapatite. Possible role in bone mineralization, bone resorption, and bone formation. Note: Gla corresponds to γ-carboxyglutamic acid.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Carter, S.D., et al. 1996. J. Anim. Sci. 74, 2719.Watson, K.E., et al. 1994. J. Clin. Invest.93, 2106.Tracy, R.P., et al. 1990. J. Bone Min. Res.5, 451.Gendreau, M.A., et al. 1989. J. Biol. Chem.264, 6972.

Packaging

100 µg in Plastic ampoule

Physical form

In 75 mM NaCl, 10 mM sodium phosphate buffer, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Sequence

H-Tyr-Leu-Asp-His-Trp-Leu-Gly-Ala-Pro-Ala-Pro-Tyr-Pro-Asp-Pro-Leu-Gla-Pro-Lys-Arg-Gla-Val-Cys²³-Gla-Leu-Asn-Pro-Asp-Cys²⁹-Asp-Glu-Leu-Ala-Asp-His-Ile-Gly-Phe-Gln-Glu-Ala-Tyr-Arg-Arg-Phe-Tyr-Gly-Pro-Val-OH (disulfide bond: 23 → 29)

Warning

Toxicity: Standard Handling (A)