Veratox® for Mustard Allergen is used for the quantitative analysis of mustard proteins in food products such as spices, sauces, dressings, meats and clean-in-place rinses. Can also be used to screen environmental swabs.
The Veratox for Mustard Allergen test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Mustard protein residue is extracted from samples with a Tris-EDTA solution by shaking in a heated water bath, followed by centrifugation or filtration. Extracted mustard protein is sampled and added to antibody-coated wells (capture antibody) where it binds to the antibody during an incubation. Any unbound mustard protein is washed away and a second antibody (detector antibody), which is enzyme labelled, is added. The detector antibody binds to the already bound mustard protein. After a second wash, substrate is added. Colour develops as a result of the presence of bound detector antibody. Red Stop reagent is added and the colour of the resulting solution is observed. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of mustard, expressed as ppm mustard.
- Accurate and easy-to-use
- Quantitate or screen samples
- High specificity
- Analyte: mustard
- Platform: ELISA
- Result Type: quantitative
- Limit of Detection: 1 ppm mustard
- Time to Result: 30 minutes
- Quantity per Package: 48 tests
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