ERK3 (MAPK6 ); actif

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Useful for kinase assay and Western blot.

Biochem/physiol Actions

Recombinant full-length human ERK3 (MAPK6) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. ERK3 (MAPK6) or mitogen-activated protein kinase 6 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases) which also known as extracellular signal-regulated kinases (ERKs) that are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals and tyrosine phosphorylated in response to insulin and NGF ⁽¹⁾. ERK3 is highly expressed in various human tissues, most abundantly in skeletal muscle ⁽²⁾.

Legal Information

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Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.