Non disponible en dehors du Royaume-Uni et de l'Irlande
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.The MISSION TagRFP Control Vector is an 8594 base pair lentivirus plasmid vector that contains a gene encoding TagRFP, under the control of the CMV promoter. The CMV-TagRFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones.
General description
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The CMV-TagRFP Control Vector is provided as 10 µg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/µl.
Legal Information
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
TagRFP is a trademark of Evrogen Co.
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