Solution d'hybridation, pour les blots du Nord et du Sud

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Suitable for use as a hybridization buffer in Northern and Southern blot protocols.

Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization.

Components

Sigma’;s Hybridization Solution contains 200 µg/mL DNA in 10x SSC and 2X Denhardt’;s solutions:200 µg/mL DNA2mg Bovine Serum Albumin (BSA)2mg Ficoll2mg Polyvinylpyrrolidone (PVP)0.15 M sodium citrate1.5 M NaCl

General description

Sigma’;s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.