MCF10A CELLS APC (-/-),

Application

The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°

Cell freezing medium-DMSO 1X, Cat. No. C6164

Media renewal changes two to three times per week.

Biochem/physiol Actions

Adenomatous polyposis coli (APC) plays an important role in cell division, adhesion and migration. Mutations in APC gene is associated with colorectal carcinomas.

Features and Benefits

These MCF10A cells are adherent with a doubling time of approx. 20 hours

Zinc finger nuclease (ZFN) knockout on chromosome 5q21

General description

MCF10A CELLS APC (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.This cell line corresponds to ATCC Catalog No. CRL-10317.Adenomatous polyposis coli (APC) is a tumor suppressor gene. It is located on human chromosome 5q21.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.