TSKgel Reversed Phase HPLC Column (Polymer-based), phase Phenyl-5PW RP, L x I.D. 15 cm x 21.5 mm, 13 mum particle size

Not available outside of the UK & Ireland.

General description

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns. Octadecyl-2PW particles have 125Å pores Octadecyl-4PW particles have 500Å pores Phenyl-5PW RP particles have 1000Å pores Octadecyl-NPR particles are nonporous

TSKgel Phenyl-5PW RP offers similiar selectivity to its hydrophobic interation counterpart TSKgel Phenyl-5PW, although a higher degree of cross-linking allows for increased stability in aqueous/organic mobile phase gradients. The highly cross-linked polymethacrylate base material provides an advantage over silica when high pH buffer systems are needed. Additionally, TSKgel Phenyl-5PW RP can withstand rigorous cleaning protocols using either acid or base. The 1000Å pore size accommodates globular protein samples up to 1,000,000 Da.

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Physical form

Shipped in 30% methanol - 70% water.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis