Colonne HPLC D'affinité TSKgel, Boronate de phase - 5 pw, l x i.d. 7,5 cm x 7,5 mm, taille des particules 10 mum

Non disponible en dehors du Royaume-Uni et de l'Irlande

General description

High efficiency, resin-based TSKgel affinity columns (10 µm particles) separate or purify many enzymes and other proteins. Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris. Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis