Non disponible en dehors du Royaume-Uni et de l'Irlande
Biochem/physiol Actions
Reversible: no
Product does not compete with ATP.
Primary TargetMonitor intracellular pH changes
Cell permeable: yes
General description
A variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines.
A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Futsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol.256, F957.
Packaging
Packaged under inert gas
1 mg in Glass bottle
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.
Warning
Toxicity: Irritant (B)
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