Anti-interleukin-12, antibody produced in goat

Not available outside of the UK & Ireland.

Application

Anti-Interleukin-12 antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. The antibody is also suitable for neutralization reactions. It has been used to neutralize IL-12 in the extracts of murine splenocytes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Interleukin-12 (IL-12) is an important cytokine that links innate and adaptive immunity. It plays a role in the regulation of Th1-associated cellular immunity to foreign pathogens, and humoral immunity to antigens that are both T cell-independent and T cell-dependent. IL-12 is primarily produced by innate immune cells, dendritic cells, epithelial cells and macrophages. All forms of IL-12 bind to the IL-12R which includes IL-12RbI and IL-12RbII subunits expressed on T cells and natural cells. IL-12 stimulates the production of IFN-γ and TNF-α, subsequent production of nitric oxide that leads to pathogen destruction. Reports suggest the involvement of JAK/STAT pathway components in mediating these effects. The effects of IL-12 have been implicated in bacterial, fungal and viral infections. Due to its role in inducing matrix metalloproteases, it also has important role in the etiology of chronic B-cell malignancies and lung adenocarcinomas Anti-Interleukin-12 antibody recognizes mouse IL-12. It shows ﹤ 10% cross-reactivity with recombinant human IL-12 and ﹤ 5% cross-reactivity with recombinant porcine IL-12.

Immunogen

purified, insect cell line Sf21-derived, recombinant mouse IL-12.

Physical form

Lyophilized from a 0.2 µm filtered solution in phosphate buffered saline containing carbohydrates.

Preparation Note

Purified using protein G.

Specificity

The antibody will neutralize the biological activity of recombinant mouse IL-12. Based on ELISA, the antibody shows ~10% cross-reactivity with recombinant human IL-12 and ﹤5% cross-reactivity with recombinant porcine IL-12.