Not available outside of the UK & Ireland.
Application
Anti-trimethyl Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody for detection of Histone H3 trimethylated on lysine 27 (H3K73me). This purified Ab is specificity verified by dot blot (DB) & validated in WB, ICC, PIA. Small pack size is ideal for screening applications.
General description
Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 lysine 27 methylation (H3K27) is associated with heterochromatin formation and transcriptional repression.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Quality
Evaluated by Western Blot in HeLa acid extract and recombinant Histone H3.
Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H3 in 10 µg of HeLa acid extract .
Specificity
Broad species cross-reactivity expected.
Target description
~ 17 kDa
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