Application
MISSION® LentiPlex® Human Pooled shRNA Library has been used to transfect human colon adenocarcinoma cell line HT29 for shRNA screening and bioinformatics analysis.
General description
MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma¢s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.The MISSION LentiPlex Human shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 75,000 shRNA constructs from the TRC collection targeting 15,000+ human genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.The library is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Each library is enough material for six screens at 100X representation.Please visit sigma.com/lentiplex to learn more and see example data and uses.
Legal Information
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
Lentiviral License Agreement
LentiPlex is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes
Deconvolution of shRNA PoolsSigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen. Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample Comprehensive, reproducible results from pooled shRNA screens Statistically robust and information-rich data Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi
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