Uracil DNA Glycosylase (UDG) is an enzyme used to eliminate carryover polymerase chain reaction (PCR) products in qPCR and RT-qPCR. This is done by incorporating dUTP in all qPCR products, so any residual products from previous PCR amplifications will contain dUs and can be digested by pre-treating with UDG enzyme.
Uracil DNA Glycosylase (UDG) catalyses the release of uracil from uracil-containing single or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases). UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200 mM).
- Optimised dTTP to dUTP ratio for high sensitivity and specificity
- High efficiency, giving excellent performance in multiplex qPCR assays
- Prevents carry-over qPCR contamination
- UDGase is also available as glycerol-free, high concentration and heat-labile
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