EO Labs g.c. Sel Agar avec plaques l.c.a.t. - 10 x 90 mm

There are a number of media available for the selective isolation of Neisseria gonorrhoeae of which this is one. Based on the medium developed by Thayer & Martin, it includes corn starch to absorb toxic metabolites and is buffered to maintain a neutral pH. The medium is made selective by the inclusion of LCAT (Lincomycin, Colistin, Amphotericin B & Trimethoprim). Lincomycin and colistin inhibit the growth of the majority of contaminating organisms likely to be present. Trimethoprim is included to reduce the spreading of Proteus spp and amphotericin B to inhibit yeasts and fungi. Yeast extract and glucose are also added as further enrichment along with laked horse blood to ensure an more luxuriant growth of Neisseria gonorrhoeae.

• Storage temperature: 2 to 8°C
• Appearance: firm gel
• Colour: cherry red
• Recommended incubation: 4 to 6% CO₂ in air for 24 to 48 hours, best results are obtained if incubation takes place in high humidity (70 to 90%)
• pH: 7.2 ± 0.2
• Plate size: 90mm