Cristaux prédéfinis Trizma, bio - formance, pH 7,4, en moyenne Mw 151,6

Code: t0319-10pak D2-231

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Open the foil pouch,...


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Votre prix
$200.58 EACH

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 °C. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature. From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.If a different concentration is desired, note that there is a concentration effect on the pH of the solution.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.

Certain pH electrodes do not give accurate results with Tris buffers.

Packaging

In convenient foil pouches

Preparation Note

Trizma solutions can be autoclaved.

application(s)diagnostic assay manufacturing
formcrystalline powder
gradeBioPerformance Certified
impurities≤5 ppm Heavy metals (as Pb), endotoxin, tested, DNase, RNase, Protease, NICKase, none detected, total aerobic microbial count, tested, ≤0.05 A290, ≤0.5% water (Karl Fischer)
mol wtaverage Mw 151.6
pH7.4, 7.12 (37 °C, 0.1 M)(lit.), 7.97 (5 °C, 0.1 M)(lit.), 7.40 (25 °C, 0.1 M)(lit.)
Quality Level200
solubilitydeionized water: soluble at 25 °C, clear, colorless (pH 7.4, 1 pouch dissolved in 1 L to yield a 0.1 M Trizma solution)
technique(s)cell culture | mammalian: suitable
useful pH range7.0-9.0
Ce produit répond aux critères suivants pour être admissible aux récompenses suivantes :



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