Anti-ZEB1

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

ControlHeLa cell lysate

Application

Research Sub CategoryTranscription Factors

Immunocytochemistry Analysis: 20 µg/mL from a representative lot detected ZEB1 in HeLa cells.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected ZEB1 in HeLa cells.

Research CategoryEpigenetics & Nuclear Function

Detect ZEB1 using this rabbit polyclonal antibody, Anti-ZEB1 Antibody validated for use in western blotting, ICC & Immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Zinc finger E-box-binding homeobox 1 (ZEB1), also known as Transcription factor 8 (TCF8), Negative regulator of IL2, and NIL-2-A zinc finger protein acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. ZEB1 has also been implicated in regulating the expression of ATP1A1, E-cadherin, BCL6, and RCOR1 genes. ZEB1 regulates neurogenesis and neuronal differentiation, but is also implicated in tumor formation. ZEB1 is expressed in heart and skeletal muscle. Mutations in ZEB1 have been associated with corneal dystrophy.

Immunogen

KLH-conjugated linear peptide corresponding to human ZEB1 .

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ZEB1 in 15 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~130 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes two isoforms at ~124 kDa