Veratox® for Hazelnut Allergen is used for the quantitative or qualitative analysis of hazelnut residues in food products such as cookies, crackers, chocolate bars, ice cream and cereals. Can also be used to screen environmental swabs.
The Veratox for Hazelnut Allergen test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Hazelnut protein is extracted from samples with a phosphate buffered salt solution (PBS) by shaking in a heated water bath, followed by centrifugation or filtration. Extracted hazelnut protein is sampled and added to antibody-coated wells (capture antibody) where it binds to the antibody during an incubation. Any unbound hazelnut protein is washed away and a second antibody (detector antibody), which is enzyme labelled, is added. The detector antibody binds to the already bound hazelnut protein. After a second wash, substrate is added. Colour develops as a result of the presence of bound detector antibody. Red Stop reagent is added and the colour of the resulting solution is observed. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of hazelnut expressed as parts per million of hazelnut.
- Accurate and easy-to-use
- Quantitate or screen samples
- High specificity
- Analyte: hazelnut, tree nuts
- Platform: ELISA
- Result Type: quantitative
- Limit of Detection: 1 ppm hazelnut
- Time to Result: 30 minutes
- Quantity per Package: 48 tests
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