Non disponible en dehors du Royaume-Uni et de l'Irlande
Application
Suitable for denaturing dsDNA before transfer to membranes during Southern procedures.
Components
The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.
General description
Sigma’;s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.
Other Notes
0.5 N NaOH, 1.5 M NaCl
Ce produit répond aux critères suivants pour être admissible aux récompenses suivantes :