MISSION Target ID Library, Easy Identification of miRNA Gene Targets

Non disponible en dehors du Royaume-Uni et de l'Irlande

Components

Library: 100µl (approx 1µg/µl), frozen liquid, store at -10°C to -25°C.Primer 1: 200µl (25µM), frozen liquid, store at -10°C to -25°CPrimer 2: 200µl (25µM), frozen liquid, store at -10°C to -25°C

General description

The Target ID Library enables bench-top transcriptome-wide human miRNA and ncRNA gene target identification. It has an innovative dual selection system, which makes rapid whole transcriptome miRNA and ncRNA gene target screens accessible to any researcher with minimal reagent, time, or capital equipment expense. The Target ID Library is a transcriptome wide cDNA pool produced using a proprietary process. Each library is tested for gene representation to ensure robust library coverage. The library is provided in ready-to-use DNA format, with enough material for multiple genome-wide screens.The Target ID Library is a pool of plasmids, each with a human cDNA inserted into the 3′-UTR region of a thymidine kinase-zeocin fusion protein (Tkzeo). Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, after introducing a miRNA or ncRNA of interest, for target cDNAs. Selected cDNAs are identified by sequencingDownload deep sequencing data of target ID library.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.