CELLULES DLD1 AKT1-/ -,

Application

Media Renenewal changes two to three times per week.Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.Subculture ratio: approx. 1:3 to 1:10.The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Cat. No. G7513, to a final concentration of 2 mM and sodium pyruvate, Cat. No. S8636, to a final concentration of 1 mM.Cell freezing medium-DMSO 1X, Cat. No. C6164.

Biochem/physiol Actions

AKT serine/threonine kinase 1 (AKT1) acts as a regulator for cell-survival and anti-apoptotic actions, which are involved in development of various cancers. This protein has a crucial role in tumorigenesis. In mice, AKT1 is essential for normal development. Mutations in the gene are associated with the pathogenesis of prostate cancer (PC). Elevated expression of the gene has been observed in human gastric cancer. Aberrations in the AKT1 and glycogen synthase kinase 3 (GSK3)-β signaling leads to the development of schizophrenia.

Features and Benefits

These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.Zinc finger nuclease (ZFN) knock out on chromosome 14q32.3

General description

DLD1 Cells SMAD4 -/- are colorectal adenocarinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.This product corresponds to ATCC Cat. No. CCL-221.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.