Deméthylase 5 C spécifique Anti-Lysine

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Research Sub CategoryHistones

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Lysine-specific demethylase 5C in 10 µg of human hippocampus tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Lysine-specific demethylase 5C in human stomach tissue.

Detect JARID using this rabbit polyclonal antibody, Anti-Lysine-specific demethylase 5C Antibody validated for use in western blotting, IP & IHC.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Lysine-specific demethylase 5C is also called Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX and Protein Xe169. Lysine-specific demethylase 5C is a histone demethylase that specifically targets trimethylated and dimethylated, but not monomethylated, ′Lys-4′ of histone H3, making it a regulator of the histone code. Lysine-specific demethylase 5C is involved in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements and is associated with Mental retardation, X-linked, syndromic, Claes-Jensen type (MRXSCJ). Lysine-specific demethylase 5C is widely expressed with highest levels found in brain and skeletal muscle.

Immunogen

KLH-conjugated linear peptide corresponding to a region near the near the N-terminus of human Lysine-specific demethylase 5C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human hippocampus tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Lysine-specific demethylase 5C in 10 µg of human hippocampus tissue lysate.

Specificity

This antibody is expected to react with all four known isoforms based upon immunogen design.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~170 kDa observed. Uniprot describes four isoforms at 176 kDa (isoform 1), 175 kDa (isoform 2), 171 kDa (isoform 3), and 155 kDa (isoform 4). Uncharacterized band(s) may be observed in some cell lysates.