Peptide de domaine liant NEMO; perméable à la cellule; contrôle négatif

Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

Reversible: no

Primary TargetNegative control defective in binding to NEMO

Product does not compete with ATP.

Cell permeable: yes

General description

A cell-permeable Antennapedia-NBD mutated (Trp⁷³⁹ and Trp⁷⁴¹ replaced with Ala) fusion peptide analog of NEMO-Binding Domain Binding Peptide (Cat. No. 480025) that serves as a negative control. Shown to be defective in binding to NEMO. Does not affect the TNF-α-induced phosphorylation of c-Jun and DNA binding of the transcription factor Oct-1.

A cell-permeable, Antennapedia-NBD mutated (Trp⁷³⁹ → Ala and Trp⁷⁴¹ → Ala) fusion peptide analog of NEMO-Binding Domain Binding peptide (Cat. No. 480025) that serves as a negative control. Reported to be defective in binding to NEMO. Does not affect the TNF-α-induced phosphorylation of c-Jun or DNA binding of the transcription factor Oct-1.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Jimi, E., et al. 2004. Nat. Med., 10, 617.May, M.J., et al. 2000. Science289, 1550.

This peptide will precipitate when added to tissue culture medium even at 100 µM concentrations; however, sufficient material will stay in solution to permeate cells.

Packaging

Packaged under inert gas

500 µg in Plastic ampoule

Sequence

H₂N-Asp-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Thr-Ala-Leu-Asp-Ala-Ser-Ala-Leu-Gln-Thr-Glu-OH

Warning

Toxicity: Standard Handling (A)