Inhibiteur de circulation des membranes; A5

Non disponible en dehors du Royaume-Uni et de l'Irlande

General description

A cell-permeable piperazinyl compound that specifically blocks clathrin adaptor complex AP-1-dependent traffic between trans-Golgi network (TGN) and endosomes in budding yeast, while exhibiting little effects against other trafficking pathways involving TGN and endosomes. Inhibits growth of yeast cells lacking complementary GGA1/2 adaptor proteins (EC₅₀ = 50 µM), but not wild-type or AP-1-deficient cells even at concentrations as high as 300 µM. A5 presumably acts at a stage after AP-1 membrane recruitment as evidenced by an enhanced AP-1 perinuclear localization upon A5 treatment of HeLa cells.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Duncan, M.C., et al. 2007. Proc. Natl. Acad. Sci. USA104, 6235.

Packaging

Packaged under inert gas

10 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)