Kit d'optimisation de chromatine Ultra Imprint, Kit conçu pour optimiser les paramètres de sonication pour les expériences de puces

Components

Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 10⁷ cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.

General description

The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and ﹤ 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.

Legal Information

Imprint is a registered trademark of Sigma-Aldrich Co. LLC