The FastGene® qFYR 384 (Plus) is a highest precision instrument for performing quantitative polymerase chain reaction (qPCR) experiments. qPCR is a well-established method for the sensitive detection and quantification of nucleic acids. During a measurement, the target DNA or RNA sequence is amplified, while a cycle-dependent increase of a fluorescent signal is detected in real-time. The FastGene® qFYR 384 (Plus) has a unique, patented optical detection system. It combines a high quality MPPC detector with a Fresnel lens that has a short focal length. The resulting short distance from detector to sample reduces signal loss and cross-talk between samples, and generally improves signal sensitivity.

The FastGene® qFYR delivers excellent temperature control, temperature precision (±0.2°C) and uniformity over the entire 96-well plate. The unique hollowed out thermal block design reduces its overall weight and ensures rapid heating and cooling rates (up to 6°C per second) for fast qPCR protocols. The system also uses state-of-the-art Peltier components for highest reaction quality and performance stability, providing reliable and consistent qPCR results.

Due to the small distance between the scanning head and the plate, the FastGene® qFYR has an outstanding level of sensitivity and virtually no cross talk between the individual wells. 4 or 6 different colour channels are installed in the scanning head to be able to excite all commonly used qPCR dyes in a single plate scan (8 sec).

The FastGene® qFYR 384 (Plus) is available in 2 different versions:

• 4+1 channels (FastGene® qFYR: PCR2544): 4 different colour channels and 1 second FAM channel
• Channel 1: FAM/SYBR Green etc.
• Channel 2: VIC/JOE/HEX/TET etc.
• Channel 3: ROX/Texas Red etc.
• Channel 4: Cy5 etc.
• Channel 5: FAM/SYBR Green etc.
• 6 channels (FastGene® qFYR Plus: PCR2546): 6 different colour channels
• Channel 1: FAM/SYBR Green etc.
• Channel 2: VIC/JOE/HEX/TET etc.
• Channel 3: ROX/Texas Red etc.
• Channel 4: Cy5 etc.
• Channel 5: Cy5.5/Quasar 705/Alexa Fluor 680
• Channel 6: ATTO 42

Features:

• Fast detection — probe and dye-based assays
• 4+1 or 6 different colour channels — perfect for multiplex qPCR (view channels)
• Standard plate format — 96-well block with gradient function
• Highest sensitivity
• Outstanding homogeneity across all wells of a 96-well plate
• Software included — easy-to-use and intuitive
• Integrated precision melt analysis tool (for probe-based allelic discrimination)
• Many applications — gene expression analysis, genotyping, HRM (high resolution melt), pathogen detection, genetic analysis
• Also available for 96-well PCR plates (FastGene® qFYR MOL2001, FastGene® qFYR Plus MOL2003)
• Accurate quantification: the broad dynamic range ensures a reliable and accurate quantification
• 1.3-fold target discrimination: smaller concentration differences can be distinguished with high accuracy, underlining the high sensitivity of the device
• Gradient qPCR for optimal annealing