TRIFLUOROACETIC ACID (25% SOLUTION IN WATER) FOR PROTEIN SEQUENATION

Not available outside of the UK & Ireland.

Analysis Note

Assay (alkalimetric): 24.5 - 25.5 %Identity (IR-spectrum): passes testDensity (d 20 °C/ 4 °C): 1.129 - 1.131Fluorescence (at 254 nm): ≤ 2 ppbFluorescence (at 365 nm): ≤ 2 ppbWater (according to Karl Fischer): 74.5 - 75.5 %

Application

Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).