Nodal is a transforming growth factor β (TGF-β) family member which has an essential role in embryonic development. Nodal signaling controls endoderm and mesoderm germ layer specification whilst preventing neuroectoderm formation. It has also been implicated in cancer metastasis. Recombinant nodal has been found to maintain pluripotency in embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). Qkine recombinant human nodal has been manufactured to be completely animal origin-free with reliable and consistent bioactivity for the reproducible maintenance of pluripotency in stem cells. Recombinant nodal is a 25.9kDa homodimer, tag and carrier free.
- High purity human nodal protein (UniProt: Q96S42)
- 25.9kDa (homodimer)
- ﹥98%, by SDS-PAGE quantitative densitometry
- Animal origin-free (AOF) and carrier protein-free
- Expressed in E. coli
- Manufactured in ISO 9001:2015 facility in Cambridge, UK
- Lyophilised from acetonitrile, TFA
- Resuspend in 10mM HCl (reconstitution solution A) at ﹥50µg/mL, add carrier protein if desired, prepare single-use aliquots and store frozen at -20°C (short-term) or -80°C (long-term)
- Featured applications: iPSC-derived mesoderm differentiation, differentiation of iPSC into endoderm, cancer research, maintenance of pluripotency in iPSC, maintenance of undifferentiated embryonic stem cells
This product has met the following criteria to qualify for the following awards: