Amyloglucosidase(A. niger) Glycerol Free 20mL

Code: FSA1862 D2-0

High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and analytical testing applications.

  • Glycerol free


 

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$306.50 EACH
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EC Number3.2.1.3
Application ExamplesRecommended for use in Total Dietary Fibre analytical procedures and the Megazyme Total Starch test method. The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method).
FormulationIn buffered solution
Physical FormSolution
CAS Number9032-08-0
Synonymsglucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase
SourceAspergillus niger
Molecular Weight143500
SpecificityHydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins
Specific Activity~ 36U/mg (40°C, pH 4.5 on soluble starch); ~400U/mL on p-nitrophenyl β-maltoside; ~6,520U/mL on soluble starch
Unit DefinitionOne unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from soluble starch (10mg/mL) at pH 4.5 at 40°C
Optimal Temperature70°C

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