Qkine Defined Bioscience HiDef-S8 400X Supplement Stem Cell Suspension Medium - Standard Vial

HiDef® S8 is a unique formulation that enables high-yield, scalable suspension expansion of human pluripotent stem cells without the need for microcarriers. The medium supports efficient aggregate formation from single-cell or small aggregate inoculation, rapid biomass accumulation, and expansion to tens of millions of PSCs per milliliter while maintaining high viability, uniform aggregate morphology, stable size distribution, and expression of key pluripotency markers. Following suspension expansion, cells readily transition back to adherent culture while preserving normal morphology and pluripotent characteristics.

HiDef® S8 contains a combination of essential nutrients and growth factors formulated specifically to maintain pluripotency while supporting robust suspension growth under agitation conditions. The addition of further components is not required, although optional surfactants such as polyvinyl alcohol (PVA), Pluronic® F-68, dextran sulfate, and others may be incorporated where desired for specific suspension workflows.

• Defined, serum-free, feeder-free medium for high-density human PSC suspension culture
• Supports microcarrier-free expansion in stirred bioreactors and other suspension systems
• Enables rapid biomass generation while maintaining high viability and pluripotency
• Compatible with single-cell inoculation and Ready-CEPT® supplementation
• Supports efficient transition back to adherent culture following suspension expansion
• Scalable from milliliter-scale research workflows to multi-liter bioreactor production