D-Lactate Colorimetric Assay, sufficient for 100 colorimetric?�tests

Not available outside of the UK & Ireland.

Application

D-Lactate Colorimetric Assay kit has been used to determine the stereospecificity of lactate produced.

General description

D-Lactate, typically present in the bloodstream at nanomolar concentrations, is produced by an intestinal source or via the methylglyoxal pathway. In mammals, D-Lactate metabolism requires D-Lactate hydrogenase and is metabolized slowly, thus an increase in blood concentration levels can lead to acidemia and acidosis. The severity of this D-lactic acidosis can be associated with neurotoxic symptoms. Significant D-Lactate accumulations in the body can also be related to impaired metabolism and excretion.

Principle

In this assay, D-Lactate is specifically oxidized by D-Lactate hydrogenase and generates a proportional colorimetric product measured at 450 nm. The useful concentration range in samples is 0.1-10 mM D-Lactate.

Suitability

Suitable for use with samples of serum, plasma, cells, culture and fermentation media.