GLUCOSE-6-PHOSPHATE ASSAY KIT

Not available outside of the UK & Ireland.

Application

Glucose-6-phosphate assay kit has been used in:glucose 6-phosphate (G6P) assayquantification of glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P)metabolite analysis

General description

Glucose 6-phosphate (G6P) is a key metabolic intermediate that enters either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase or glucokinase or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. G6P lies at the beginning of both glycolysis and the pentose phosphate pathways. It also can be stored as glycogen when blood glucose levels are high.

Principle

G6P is utilized by Glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. G6P is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the G6P present. Typical sensitivities of detection are between 1-30 nmoles.

Suitability

Suitable for quantifying Glucose-6-Phosphate in a variety of samples.