Anti-Protein A antibody produced in rabbit, fractionated antiserum, lyophilized powder

Not available outside of the UK & Ireland.

Application

Anti-Protein A antibody produced in rabbit has been used in immunoblotting.

Protein A may be used as in immunoblotting with a minimum dilution of 1:50,000.

Biochem/physiol Actions

Protein A has an ability to bind Fc region of immunoglobulins from several species. It is used as a “universal tracer” and it facilitate the segregation of bound and free ligand. Protein A also acts as affinity matrices for antibody (IgG) purification.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. To ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary. Rabbit Anti-Protein A is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, to which no preservatives have been added. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG results in a single arc of precipitation and versus anti-rabbit whole serum results in multiple arcs of precipitation.

Protein A is a 42 kDa bacterial protein. It is isolated from the cell wall of Staphylococcus aureus.

Immunogen

Protein A from Staphylococcus aureus

Physical form

Lyophilized from 0.01M phosphate buffered saline, pH 7.2

Reconstitution

Reconstitute with 2 ml deionized water.