X-phos-MAPK 1/2 (T202/Y204) cl 14B9.1

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

ControlA431cell lysate and EGF treated A431 cell lysate

Application

Research CategorySignaling

Research Sub CategoryDevelopmental Signaling

Anti-phospho-MAPK 1/2 (Thr203/Ty205) Antibody, clone 14B9.1 is a highly specific mouse monoclonal antibody & that targets MAPK/Erk1/2 & has been tested in western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. The 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade. For this reason, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The ERK1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Aberrant control of ERK1/2 activity has been implicated in a variety of pathological conditions, including cancer and autoimmune diseases, and efficient study methods are in demand. Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.

Immunogen

KLH-conjugated linear peptide corresponding to human MAPK 1/2 phosphorylated at Thr203 and Ty205.

Linkage

Replaces: 05-481

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected phospho-MAPK 1/2 (Thr203/Ty205) in 200 µg of A431cell lysate and EGF treated A431 cell lysate.

Specificity

Antibody recognizes dual phosphorylation of MAPK1/2 at Thr203/Tyr204, but this antibody has not been used for the detection of single phosphorylation at either site.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 44 kDa observed