Souris Anti-OCLN mAb (1G7)

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Positive ControlHL-60 cells, HeLa cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)

General description

This Anti-OCLN Mouse mAb (1G7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of OCLN.

Purified mouse monoclonal antibody. Recognizes the ~55-60 kDa OCLN protein.

Recognizes the ~55-60 kDa OCLN protein in HL-60 and HeLa cells.

Immunogen

A recombinant polypeptide corresponding to amino acids of 423-523 human OCLN, expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)