Anti-ABI1 antibody produced in rabbit, approx. 1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Not available outside of the UK & Ireland.

Application

Rabbit anti- ABI1 antibody can be used for immunoblotting assays at a concentration of 0.5-1.0µg/ml using extracts from A431 and rat brain cells.

Anti-ABI1 antibody produced in rabbit has been used in western blot analysis and immunofluorescence.

Biochem/physiol Actions

Abelson interactor 1 (Abi1) is essential for the formation and activation of WAVE2 signaling complex. ABI1 and WAVE are essential for Rac-dependent membrane protrusion and macropinocytosis, whereas ABI1 and N-WASP regulate actin-based vesicular transport, EGF receptor endocytosis, and EGFR and transferrin receptor (TfR) cell surface distribution. ABI1 has been shown to act as dual regulator of WAVE and neural Wiskott-Aldrich syndrome protein (N-WASP) specific activities. ABI1 is an essential component of this signaling complex. It positively regulates WAVE activity and connects WAVE to Rac through assembly of the WAVE-ABI1-Nap1-PIR121 complex. Abi1 modulates actin reorganization and lamellipodia formation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Abi 1 is an adaptor protein that interacts with Wave2 to regulate actin polymerization.

Abelson interactor 1 (Abi1) protein, a 65 kD-substrate of the eponymous Abelson non-receptor tyrosine kinase (c-Abl) is expressed mainly in the central nervous system. This gene is located on human chromosome 10p12.

Immunogen

synthetic peptide corresponding to amino acids 163-180 of human ABI1, conjugated to KLH. The corresponding sequence is identical in several species including mouse, rat, and dog.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.