ANTI-PRODYNORPHIN(B29),CL DYN29

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

This mouse monoclonal Anti-Prodynorphin (Dynorphin B29), clone Dyn9 Antibody, Cat. No. MABT882 is validated for use in Immunohistochemistry (Paraffin) for the detection of prodynorphin and leumorphin/dynorphin B-29.

General description

Proenkephalin-B (UniProt P06300; also known as Beta-neoendorphin-dynorphin, Preprodynorphin) is encoded by the Pdyn gene (Gene ID 29190) in rat species. Proenkephalin B is an opioid polypeptide hormone involved with chemical signal transduction and cell communication. Prodynorphin is a basic building-block of endorphins, the chemical messengers in the brain that appear most heavily involved in the anticipation and experience of pain and the formation of deep emotional bonds critical in learning and memory. Prodynorphin is produced with a signal peptide (a.a. 1-21) and a propeptide (a.a. 22-163) sequence. Following the removal of the signal and propeptide sequences, the protein is further processed by proeolytic cleavages to form Alpha-neoendorphin (a.a. 166-175), Beta-neoendorphin (a.a. 166-174), Big dynorphin (a.a. 202-233; Big Dyn), Dynorphin A(1-17) (a.a. 202-218; Dyn-A17; Dynorphin A); Dynorphin A(1-13) (a.a. 202-214), Dynorphin A(1-8) (a.a. 202-209), Leu-enkephalin (a.a. 202-206 & 221-225), Leumorphin (Dynorphin B-29) (a.a. 221-248), and Rimorphin (a.a. 221-233; Dynorphin B; Dyn-B; Dynorphin B(1-13)).

Immunogen

Thyroglobulin-conjugatged linear peptide corresponding to rat leumorphin (dynorphin B-29) C-terminal half sequence.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Immunohistochemistry in human thalamus tissue sections.

Immunohistochemistry Analysis: A 1:20 dilution of this antibody detected Prodynorphin/Dynorphin B29 immunoreactivity in human thalamus tissue sections.

Specificity

Clone Dyn9 recognizes preproenkephalin-B (a.a. 1-248 of rat sequence), proenkephalin-B (a.a. 22-248 of rat sequence), and leumorphin/dynorphin B-29 (a.a. 221-248 of rat sequence), but not other peptides derived from Proenkephalin-B.

Target description

28.08/28.06/28.39 kDa (Rat/Mouse/Human preproenkephalin-B), 25.85/25.76/26.28 kDa (Rat/Mouse/Human proenkephalin-B), 3.320/3.320/3.352 kDa (Rat/Mouse/Human leumorphin) calculated.