Monoclonal Anti-Granulocyte Colony Stimulating Factor antibody produced in rat, clone 67604, purified immunoglobulin, lyophilized powder

Not available outside of the UK & Ireland.

Application

Anti-granulocyte colony stimulating factor antibody may be used for immunoblotting at a working concentration of 1-2 µg/ml. For ELISA the recommended concentration range is 2-8 µg/ml. The antibody is also suitable for neutralization assays.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mature myeloid cells are derived from bone-marrow derived precursor cells by the activity of three colony stimulating factors, granulocyte/macrophage colony-stimulating factor (GM-CSF), macrophage colony-stimulating factor (M-CSF) and granulocyte colony-stimulating factor (G-CSF). The expression of G-CSF is the result of activation of pathways in response to tumor necrosis factor-α, LPS and toll-like receptor ligands. The pathways that induce the effects of G-CSF are PI3K, ERK1/2 and p38. The generation of neutrophils and induction of the circulation of hematopoietic stem cells from the bone marrow are the main functions of G-CSF. There are additional activities attributed to G-CSF such as neuroprotection, immunomodulation and generation of cardiac cells and dendritic cells. Large quantities of G-CSF are secreted in a variety of myeloid and non-myeloid cancers and rheumatoid arthritis Monoclonal anti-granulocyte colony stimulating factor recognizes mouse G-CSF. The antibody shows less than 0.06% cross-reactivity with recombinant human G-CSF.

Immunogen

purified, recombinant mouse granulocyte colony stimulating factor, expressed in E. coli.

Physical form

Lyophilized from a 0.2 µm filtered solution in phosphate buffered saline with 5% trehalose.