PhosphoDetect# Anti-PDH-E1# 1PC X 50UG

Not available outside of the UK & Ireland.

Analysis Note

Negative ControlHEK293 cells treated with dichloroacetate

Positive ControlHEK293 cells

Application

Immunoblotting (0.5 µg/ml)Immunocytochemistry (1 µg/ml)

General description

Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser³⁰⁰ in HEK293 cells.

This PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of PDH-E1α (pSer³⁰⁰).

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser³⁰⁰.

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.Seifert, F., et al. 2007. Biochemistry 21, 6277.Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

Due to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)