Non disponible en dehors du Royaume-Uni et de l'Irlande
Application
Anti-Omentin Antibody detects level of Omentin & has been published & validated for use in ELISA & WB.
Western blot: 1:2,000-1:5,000 using ECL. Reacted with the ~40kDa Omentin His-tagged protein. Suggested blocking and dilution buffer is PBST with 0.05% Tween-20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
ELISA, direct and indirect: 1:2,000-1:5,000.
General description
A calcium-dependent lectin with affinity for furanosides, such as galactofuranosyl in bacterial arabinogalactans. 30kD of apparent molecular weight of Omentin migrate at 40-kD under reducing conditions due to glycosylation. Omentin, a novel visceral fat depot-specific secretory protein, enhance insulin-stimulated glucose transport in subcutaneous as well as omental adipocytes and may therefore be implicated in the pathogenesis of obesity.
Immunogen
Epitope: Amino acids 95-115 of Human Omentin.
Synthetic peptide: TVGDRWSSQQGSKADYPEGDG, corresponding to amino acids 95-115 of Human Omentin.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Liquid in PBS, pH7.4. Contains no preservatives.
Specificity
Recognizes Omentin in western blotting.
Target description
30-40 kDa
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