Not available outside of the UK & Ireland.
Application
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
Goat Anti-Biotin-Alkaline Phosphatase antibody has been used for dot blot assays. The product can also be used for ELISA (1:30,000) and immunohistochemistry (1:50).
ELISA Assays using human spleen cells were performed using alkaline phosphatase-conjugated goat anti-biotin as the secondary antibody with incubation for 1.5 hours at room temperature. Purified IgG from K/BxN, BxSB or C57BL/6 mouse serum was analyzed by western blot using alkaline phosphatase-conjugated goat anti-biotin antibody as the secondary.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Biotin is a carboxylase co-enzyme that regulates the metabolism of amino acids, glucose and fatty acids. This co-enzyme has been implicated in processes such as immune responses, cell division and fetal growth. Biotin may also be involved in the epigenetic modifications of chromatin.Goat Anti-Biotin-Alkaline Phosphatase antibody is specific for biotin when tested against biotin-goat IgG, goat IgG, and KLH.
Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.
Immunogen
Biotin-KLH conjugate
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide
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