Anti-CD40 Mouse mAb (EA-5)

Not available outside of the UK & Ireland.

Application

Flow cytometry (5 µg/ml)r>Frozen Sections (see comments)r>Immunoprecipitation (see comments)r>Paraffin Sections (see comments)

General description

Recognizes the ~44 kDa CD40 protein in Raji cells.

Protein A purified mouse monoclonal antibody. Recognizes the ~44 kDa CD40 protein.

Anti-CD40, mouse monoclonal, clone EA-5, recognizes ~44 kDa CD40 in Raji cells. It is validated for use in Flow Cytometry, immunoprecipitation, & immunohistochemistry on frozen & paraffin sections.

Immunogen

transfected murine L cells expressing human CD40

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Fanslow, W.C., et al. 1992. J. Immunol.149, 655.Law, C.L., et al. 1990. Leukemia4, 732.

FACS was performed on 5x10⁵ cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.

Warning

Toxicity: Standard Handling (A)