c-Kit (V560G)

Not available outside of the UK & Ireland.

General description

N-terminal GST tagged recombinant human c-Kit, amino acids 544 - end, containing the V560G mutation. The V560G mutation has been found in patients with gastrointestinal stromal tumours and has been linked to poor prognosis. This mutation confers increased susceptibility to Gleevec™.

Product Source: Human c-Kit (V560G) expressed in Sf21 insect cells.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Physical form

Presented as a frozen solution in 50mM Tris/HCl pH 7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, and 0.1% 2-mercaptoethanol.

Quality

Routinely evaluated by phosphorylation of 250µM GGMEDIYFEFMGGKKK.