Neogen Veratox VIP for Peanut ELISA Allergen Test Kit - 48 Tests

Veratox® VIP (Veratox Improvement Platform) for Peanut Allergen offers enhanced quantitative analysis of very low levels of raw and further-processed peanut residues in a wide variety of food products, ingredients, and clean-in-place rinses. This product can also be used to screen environmental swabs.

The Veratox VIP for Peanut test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). This assay is validated to detect three primary allergenic proteins in peanuts (Ara h 1, Ara h 2, and Ara h 3). Peanut protein is extracted from samples with a phosphate-buffered salt (PBS) based solution by shaking in a heated water bath, followed by centrifugation or filtration. Extracted peanut protein is sampled and added to antibody-coated wells (capture antibody), where it binds to the antibody during an incubation. Any unbound peanut protein is washed away and a second antibody (detector antibody), which is enzyme labelled, is added. The detector antibody binds to the already bound peanut protein. After a second wash, substrate is added. Colour develops as a result of the presence of bound detector antibody. Red Stop Solution reagent is added and the colour of the resulting solution is observed. The test is read in a microwell reader to yield optical densities (OD). The OD of the controls form a standard curve, and the sample OD are plotted against the curve to calculate the concentration of peanut protein.

• Features an optimised antibody combination and extraction procedure for high specificity to all types of peanut protein, particularly further-processed peanut
• Highly sensitive, quantifying peanut protein down to 0.25 ppm, aligned with most up-to-date industry guidelines
• Rapid and easy to use, with a 30 minute time to result, a simple testing procedure consistent across sample types, and ready to use, non-hazardous reagents
• Analyte: peanut
• Platform: ELISA
• Result Type: quantitative
• Limit of Detection: 0.08 ppm peanut protein
• Range of Quantitation: 0.25 ppm peanut protein - 5.00 ppm peanut protein
• Time to Result: 30 minutes
• Quantity per Package: 48 tests