Veratox® for Egg Allergen is used for the quantitative analysis of food products and clean-in-place rinses. Can also be used to screen environmental swabs.
Veratox for Egg Allergen is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Egg protein residue is extracted from samples with a buffered salt solution (Phosphate Buffered Saline, or PBS) by shaking in a heated water bath, followed by centrifugation or filtration. Extracted egg protein is sampled and added to antibody coated wells (capture antibody) where it binds to the antibody during an incubation. Any unbound protein is washed away and a second antibody (detector antibody), which is enzyme labelled, is added. The detector antibody binds to the already bound egg protein. After a second wash, the substrate is added. Colour develops as a result of the presence of bound detector antibody. Red Stop reagent is added and the colour of the resulting solution is observed. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of egg allergen.
- Accurate and easy-to-use
- Cost effective microwell format
- High specificity
- Analyte: egg
- Platform: ELISA
- Result Type: quantitative
- Limit of Detection: 0.6 ppm whole dried egg
- Time to Result: 30 minutes
- Quantity per Package: 48 tests
This product has met the following criteria to qualify for the following awards: