Kit de protection HPLC de TSKgel Affinity, phase Boronate - 5 pw, l x i.d. 2,5 cm x 6 mm, taille des particules de 20 mum

Non disponible en dehors du Royaume-Uni et de l'Irlande

Components

TSKgel Guardgel kits contain: 5 mL Guardgel packing material Column holder, consisting of external reducing union, cap, frit, 2 ferrules Column, 25 mm x 9.5 mm OD x 6 mm ID, 2 µm frit at 10 mm depth, 1/16" 2 Replacement frits Capillary tubing, 5 cm x 1/16" OD x 0.01" Nuts and ferrules, 10-32 threads for 1/16" tubing, 2 each All hardware is 316 stainless steelDimensions for Guardgel kits for 21.5 mm ID columns: Column, 35 mm x 12.75 mm OD x 10 mm ID, 10 µm frit at 20mm depth, 1/16" Capillary tubing, 5 cm x 1/16" OD x 0.02" ID

General description

Guardgel Kit for 7.5mmID TSKgel Boronate-5PW columnsHigh efficiency, resin-based TSKgel affinity columns (10 µm particles) separate or purify many enzymes and other proteins. Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris. Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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