Non disponible en dehors du Royaume-Uni et de l'Irlande
Biochem/physiol Actions
Product does not compete with ATP.
Primary TargetHh signaling in Shh-light2 assay
Cell permeable: yes
General description
Cyclopamine, V. californicum, CAS 4449-51-8, is a cell-permeable steroidal alkaloid & cholesterol mimic that specifically antagonizes Sonic Hedgehog signaling through direct interaction with Smo.
A cell-permeable steroidal alkaloid and cholesterol mimic that displays both teratogenic and antitumor activities. It disrupts cholesterol bio-synthesis and specifically antagonizes shh (Sonic Hedgehog) signaling pathway through direct interaction with Smo (smoothened), a distant relative of G-protein-coupled receptors. A valuable tool for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control. Also available as a 10 mM solution in Ethanol (Cat. No. 239806).
A cell-permeable, steroidal alkaloid and cholesterol mimic that displays both teratogenic and anti-tumor properties. Disrupts cholesterol biosynthesis and specifically antagonizes shh (Sonic Hedgehog) signaling through direct interaction with Smo (smoothened), a protein that is related to G-protein-coupled receptors. Useful for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Berman, D.M., et al. 2003. Nature,425, 846.Thayer, S.P., et al. 2003. Nature,425, 851.Watkins, D.N., et al. 2003. Nature422, 313.Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.Berman, D.M., et al. 2002. Science297, 1559.Taipale, J., et al. 2000. Nature406, 1005.Kim, S.K., and Melton, D.A. 1998. Proc. Natl. Acad. Sci. USA95, 13036.Cooper, M.K., et al. 1998. Science280, 1603.Incardona, J.P., et al. 1998. Development125, 3553.
Packaging
1, 5 mg in Glass bottle
Preparation Note
Ethanol stock solutions may require warming to achieve complete solubilization.
Reconstitution
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Warning
Toxicity: Carcinogenic / Teratogenic (D)
Ce produit répond aux critères suivants pour être admissible aux récompenses suivantes :