Not available outside of the UK & Ireland.
Application
SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.
Features and Benefits
Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP. Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming. No need to fragment DNA before sequencing. Amplifies ds-cDNA in 8 hours or less. Compatible with all next generation sequencing platforms except Pacific Bioscience.
General description
The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.
Legal Information
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
Other Notes
SEQR-500RXN is manufactured on-demand. Contact us for more information.
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